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Old 23-05-2015, 03:37 PM   #1
MyQul
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Default How to culture up yeast from bottle conditioned beers

This guide is if you dont have a stir plat or erlenmeyer flask

You'll need:

1x500ml water bottle
2x 2L water bottles
Bag of DME
Star San in a spray bottle

Put the bottle your going to havest yeast from in the fridge for a few days so as much yeast as possible is crashed out at the bottom. Then carefull pour off your beer (all in one go) leaving about 1-2cm of beer and hopefully yeast, in the bottom of the bottle

I usually do 5 'step ups' to take me from from yeast dregs to pitchable amount.

Step up 1. Make 100ml of 1.020 wort using the DME. You need to boil/simmer for 15 mins to sanitise. For this stage you might want to make 3 times as much as simmering 100ml o wort for 15 mins tends to cause the pot to boil dry - put this in the 500ml bottle with the dregs from your bottle When you pour the dregs from the bottle spray with starsan. Shake the bottle to aerate and leave for 3 days somewhere warm (at least room temp). You need to keep shaking the bottle about 4 times per day. Once shook loosen the lid a little to allow CO2 to escape.Make sure you remember to re-tighten the lid before each shake or it's not going to be pretty

Step up 2. Make another 100ml of 1.020 wort - add this to the 500ml bottle. Shaking 4 times per day for 3 days. Make sure you remember to tighten the lid. When you open the bottle to pour in the new wort, spray around the lid before opening and also around the mouth of the bottle

Step up 3. Transfer everything from the 500ml bottle to one of the 2L bottles and add 250ml of 1.040 wort. Shake 4 times per day for 2/3 days. Spray starsan around both bottle opening before and after you take the lids off

After 2/3 days put the 2L bottle in the fridge for 1-3 days until all the yeast has crashed out of solution and is at the bottom of the bottle. Then pour off most of the liquid/spent wort and shake the compacted yeast to resuspend in the remaing bit of liquid. Then pour half into the second 2L bottle. Spray starsan around both bottle opening before and after you take the lids off

Step up 4. Make 2L of 1.040 wort and put 1L in each bottle. Shake 4 times per days for 2/3 days. Spray starsan around both bottle opening before and after you take the lids off

The reason you use 2 bottles is than when you shake the bottles they really foam up because of all the C02 created by the yeast. When you open the lid to let the C02 out, if you were to just use 1 2L bottle there wouldn't be enough head room for all the foam. Tbh, there still isn't enough head room with 1L in a 2L bottle so after shaking you usually need to leave it for 30 mins for the foam to calm down a bit else loads of foam/yeast spurts out.

Step up 5. Put the bottles in the fridge for 1-3 days to crash the yeast then pour off the spent wort when the yeast is compacted. Take the bottles out the fridge allow to come to room temp and add 1L of 1.040 wort to each bottle. Shake 4 times per day for 2/3 days. You should now have a pitchable amount of yeast. You can crash again in the fridge but you dont have to (but I do).

This all takes about 3 weeks to 'grow' enough yeast for an average (1.040ish OG) beer.

DME Amounts:

100ml/1.020 = 5g DME
250ml/1.040=25g DME
1L/1.040 = 100g
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Old 06-12-2015, 08:35 PM   #2
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How many bottles of the original beer is needed?
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Old 06-12-2015, 08:39 PM   #3
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I used one bottle of Butty Bach to get the Wye Valley yeast and there was a trace in the bottle. Over two and a half weeks though it turned into 100ml!
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Old 06-12-2015, 08:52 PM   #4
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The above is for one bottle of beer. You'll usually just get a miniscule amout which you can the culture up
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Old 07-12-2015, 12:27 PM   #5
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Ok thanks. Only have 330ml bottles of the ones I want to try. Need to get some dme before I can start drinking. I am not sure if they are primary strains, I think two of the 6 bottles have wine yeast in it
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Old 11-05-2016, 07:27 AM   #6
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Trying this now on the SN.. I am sad enough to even bring the bottle to work with me.. I can see the black trub buts which I assume is the yeast perhaps dead yeast..

I swirl shake a lot but apart from bubbles rising but I do not see a lot in the way of yeast just flakes at the bottom.. I did wonder whether that might be because of the carbonation in the original beer but it would be flat now wouldn't it.
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Old 11-05-2016, 06:39 PM   #7
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Trying this now on the SN.. I am sad enough to even bring the bottle to work with me.. I can see the black trub buts which I assume is the yeast perhaps dead yeast..

I swirl shake a lot but apart from bubbles rising but I do not see a lot in the way of yeast just flakes at the bottom.. I did wonder whether that might be because of the carbonation in the original beer but it would be flat now wouldn't it.
The first 1 or 2 step ups I usually dont see anything really. The only way I know anything is happening is that at step up 2 or 3 I can hear the off gassing of the C02
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Old 11-05-2016, 07:01 PM   #8
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SN?

Sierra Nevada?

I've seen these flakes on the bottom of the bottle. Thought how weird it was.
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Old 11-05-2016, 07:04 PM   #9
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SN?

Sierra Nevada?

I've seen these flakes on the bottom of the bottle. Thought how weird it was.
Close but no banana. Shepard Neame
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Old 11-05-2016, 07:04 PM   #10
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Great post MyQul

I'm going to give this a try with a kernel brewery beer. Just have to get down to the bottle shop.

But I'm off next Monday

I have a stir plate and a flask. So I think I will try a both methods.

I'll just have to buy two bottles
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