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Of course I'm more than happy to help out where I can, no problems :cheers:

Maybe so, however if you give it a go you might get a reasonable out of freeze viability just using a simplified method, definitely worth a shot though,... :wha: The stuff I used to do was v.anal as the cells were manufacturing cell lines i.e. these were the fermentation starter cultures that...

you could always buy a very fast hamster and turn its wheel into a DIY centrifuge :hmm: sorry, i'm not being very helpful am I.. :doh:

just quickly googled and found this, I'm guessing nobody had liquid nitrogen or a -80c freezer at home so will have to experiment with a -20 and see how it works. Freezing yeast Materials:
yeast culture
DMSO
glycerol
water Protocol: 1. Take 5 ml yeast cells from the Erlenmeyer of...

I used to work as a scientist in cell line development, part of this was laying down cell line banks for long term storage. One of the chemicals that we used as a cryoprotectant was DMSO, Dimethyl sulfoxide, It prevents the crystalization of water, this is an issue during the freeze thaw...

GraysAlchemy - I think you mean Pink with Sequins Yes there does seem to be a shortage of girls on here. (note to self; start an underground female brewing mafia set for world domination) I have to admit though while I brew the wine and cider for myself the beer is in fact all for my Dad. My...

Hi, I hope this isn't a silly question but I've recently ordered a load of bored cork bungs for DJ's.... put one in with an airlock etc the problem is that the wine is clearly fermenting, there's a LOT of bubble activity but the airlock isn't bubbling at all,... I checked the airlock for leaks...

Oooh, that's very interesting. I've been thinking about getting a raspberry pi for christmas,.. I think it would take me a while to get to the fermenter controller stage (I'm currently learning java but haven't got much further yet) I did used to work as a scientist and work with mammalian...

Oooh,... hand't even thought of this,.. i'm in north Cambridge. I have heard about people using bottled water to brew or treating it, does it make a huge amount of difference to the process or just subtle flavour?

Hello, I've been reading a few things on here and benefitting from everyone's good advice so I thought I should register and hopefully be able to partake in the discussion a bit. I've brewed a few cider kits before 2xYoungs cider 1x magnum apple cider And 2 woodforde's beer kits: Wherry &...